Banded chromosome images recognition based on dense convolutional network with segmental recalibration / 生物医学工程学杂志

Human chromosomes karyotyping is an important means to diagnose genetic diseases. Chromosome image type recognition is a key step in the karyotyping process. Accurate and efficient identification is of great significance for automatic chromosome karyotyping. In this paper, we propose a model named segmentally recalibrated dense convolutional network (SR-DenseNet). In each stage of the model, the dense connected network layers is used to extract the features of different abstract levels of chromosomes automatically, and then the concatenation of all the layers which extract different local features is recalibrated with squeeze-and-excitation (SE) block. SE blocks explicitly construct learnable structures for importance of the features. Then a model fusion method is proposed and an expert group of chromosome recognition models is constructed. On the public available Copenhagen chromosome recognition dataset (G-bands) the proposed model achieves error rate of only 1.60%, and with model fusion the error further drops to 0.99%. On the Padova chromosome dataset (Q-bands) the model gets the corresponding error rate of 6.67%, and with model fusion the error further drops to 5.98%. The experimental results show that the method proposed in this paper is effective and has the potential to realize the automation of chromosome type recognition.

Separation and Enrichment of Antibacterial Effective Fraction of Syringae Folium by Macroporous Resins / 中国药师

Objective:To investigate the purification of antibacterial effective fraction of Syringae folium by macroporous resins. Methods:Static adsorption and desorption tests were carried out to screen the macroporous resins. The desorption experiment was per-formed on the selected D101 resin to optimize the separation process. The effects of resin amount, diameter length ratio, elution flow rate, elution solution concentration and volume were studied. Results:The optimal conditions were as follows:the elution solution was 55% ethanol, the adsorption flow rate was 1 BV·h-1 , the elution flow rate was 5 BV·h-1 , 6 BV 25% ethanol was used to eliminate impurity and 8 BV 55% ethanol was used to elute to obtain the effective fraction. Conclusion: The content of antibacterial effective component is above 65% after purified by D101 resin, indicating that the present method is suitable for large-scale preparation of anti-bacterial effective fraction of Syringae folium.

Effects of arsenic trioxide combined with cisplatin on expression of PCNA and Ki-67 in human nasopharyngeal carcinoma xenograft in nude mice / 中国耳鼻咽喉头颈外科

[ABSTRACT]OBJECTIVETo study the effects of Arsenic Trioxide on the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, and explore the possible mechanisms of the antitumor effect. METHODSThe models of human poorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into 4 groups (control group, As2O3 group, DDP group and As2O3+DDP group). The antitumor effect of every group was studied. Pathological changes of tumor tissues dyed by HE staining were observed with light microscope. The pathological changes of the heart, liver, lung and kidney from nude mice were also observed by HE staining. Blood routine test was examined. Expression of PCNA and Ki-67 were detected by immunohistochemistry method.RESULTSThe growth of tumor in As2O3, DDP and As2O3+DDP were obviously inhibited. No pathological changes and metastases were found in heart, liver, lung and kidney of the nude mice. No obvious toxicity of the treatment to the hematopoietic systems in the nude mice was observed. The expression of PCNA and Ki-67 were down-regulated.CONCLUSIONAs2O3 can inhibit the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, the mechanism of which might be related to the down-regulation of PCNA and Ki-67.

The influence of hypothermia plasma radiofrequency ablation on synechia nasal after nasopharyngeal carcinoma radiotherapy / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To observe the effect of hypothermia plasma radiofrequency ablation to the adhesion of nasal cavity after radiotherapy of nasopharyngeal carcinoma.@*METHOD@#The subjective score combined with nasal ventilation function test were used to reflect the degree of patients with nasal adhesion.@*RESULT@#There is a significant improvement in subjective feeling after treatment. Nasal cavity volume began to increase and nasal expiratory resistance decrease obviously 3M later.@*CONCLUSION@#Hypothermia plasma radiofrequency ablation technology can improve the nasal cavity adhesion in patients with nasopharyngeal carcinoma after radiotherapy, and also the patients quality of life.

Effects of arsenic trioxide combined with cisplatin on the growth of human nasopharyngeal carcinoma cells and reversion of RASSF1A hympermethylation / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the effect of arsenic trioxide (As2O3) combined with cisplatin on expression of RASSF1A in nude mice with human nasopharyngeal carcinoma xenograft.@*METHOD@#The models of human poorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into four groups, control group (NaCl group), As2O3 group, DDP group and As2O3 + DDP group. The expression of RASSF1A mRNA and protein were detected by Real-time RT-PCR and immunohistochemistry respectively. The methylation rate of RASSF1A promoter CpG islands was analyzed by HRM.@*RESULTS@#Experimental groups could obviously inhibit the growth of tumor and up-regulate the expression of RASSF1A. The methylation rate of RASSF1A in transplanted tumors in experimental groups was lower than the control group. Especially As2O3 combined with DDP were superior to the single drug use.@*CONCLUSION@#As2O3 inhibits the growth of human nasopharyngeal carcinoma cell strain CNE2 xenograft in nude mice and increases mRNA expression of RASSF1A. As2O3 inhibits the malignant phenotypes of human nasopharyngeal carcinoma cells and reverses hypermethylation of RASSF1A.

Inhibitory effect of arsenic trioxide combined with cisplatin on human nasopharyngeal carcinoma xenograft and DAPK in nude mice / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the inhibitory effect of Arsenic Trioxide (As2O3) combined with diamminedichloroplatinum (DDP) on the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, and to explore the possible effect mechanisms of the antitumor.@*METHOD@#The models of human poorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into four groups, control group, As2O3 group, DDP group and As2O3 + DDP group. The effect of antitumor on each group was studied. The specimen obtained from the mice were detected by optical microscope and tdt-mediated dutp rock end labeling (tunel) method. Expression of DAPK was detected by real time-PCR and immunohistochemistry.@*RESULT@#As2O3 group and AS2O3 + DDP group could obviously inhibit the growth of tumor, induce the apoptosis of human naso pharyngeal carcinoma cell and up-regulate the expression of RASSF1A.@*CONCLUSION@#As2O3 can greatly inhibit the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, which were related to the induced apoptosis of human nasopharyngeal carcinoma cell and up-regulated expression of DAPK Combination of As2O3 with DDP seem to be more effective.

Apoptosis of nasopharyngeal carcinoma cells line CNE-2 induced by dihydroartemisinin and its possible mechanism / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#Dihydroartemisinin is a traditional anti-malarial drug, a derivative of the artemisinin, it has anti-tumor activity of a variety of tumor cells. This study investigated the effect of growth inhibition of nasopharyngeal carcinoma cells line CNE-2 induced by dihydroartemisinin and its possible mechanism.@*METHOD@#The effect of DHA on the cell proliferation of CNE-2 was detected by CCK-8 assay with different concentrations and time. The effects of DHA on the cell apoptosis of CNE-2 were detected by Annexin V-FITC assay through flow cytometry and caspase-3 activity assay.@*RESULT@#CCK-8 experimental results show that CNE-2 cell proliferation was suppressed with DHA treatment, as compared with the control group. DHA could induce marked apoptosis in CNE-2 by apoptosis assay, as compared with the control group, The percentages of apoptotic cells increased along with the increase of DHA concentrations in CNE-2; The activity of caspase-3 was increased following DHA treatment in a dose-dependent manner.@*CONCLUSION@#DHA could effectively inhibit proliferation and induce apoptosis of human nasopharyngeal carcinoma cells line CNE-2, the possible mechanism DHA induce apoptosis of CNE-2 cells by upregulating the expression of caspase-3.

Experimental research on the relationship between GATA-3 and ICAM-1 in patients with allergic rhinitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the relationship between GATA-3 and ICAM-1 in patients with allergic rhinitis.@*METHOD@#The expression of GATA-3 was detected by reverse transcriptase polymerase chain reaction (RT-PCR) in 37 patients with allergic rhinitis and 12 controls. ICAM-1 was determined by enzyme-linked immunosorbent assay.@*RESULT@#The relative density ratio of GATA-3 to GAPDH in AR patients was 0.632 +/- 0.121, and in control group was 0.131 +/- 0.024. The concentrations of ICAM-1 were (237.5 +/- 37.3) pg/mg and (121.7 +/- 22.4) pg/mg in the two groups respectively. The expression of GATA-3 was positively correlated to the expression of ICAM-1 in patients with allergic rhinitis.@*CONCLUSION@#The overexpression of GATA-3 in patients with allergic rhinitis was related to the increase of ICAM-1. GATA-3 may play an important role in the pathogenesis of allergic rhinitis.

Relationship between GATA-3 and IL-12 in patients with allergic rhinitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the relationship between GATA-3 and IL-12 in patients with allergic rhinitis.@*METHOD@#The expression of GATA-3 was detected by reverse transcriptase polymerase chain reaction (RT-PCR) in 37 patients with allergic rhinitis and 12 control samples. IL-12 was detected by enzyme-linked immunosorbent assay.@*RESULT@#The relative density ratio of GATA-3 to GAPDH in AR patients was 0.579 +/- 0.102, and in control group was 0.128 +/- 0.021. The concentrations of IL-12 were (53.7 +/- 12.3) ng/g and (121.9 +/- 20.4) ng/g in the two groups respectively. The expression of GATA-3 was negatively correlated to the expression of IL-12 in patients with allergic rhinitis.@*CONCLUSION@#The overexpression of GATA-3 in patients with allergic rhinitis was related to the decrease of IL-12, and to increasing the expression of IL-12 in topical tissue could suppress the expression of GATA-3 and may improve the therapeutic effectiveness of AR.

Effects of procaine on human nasopharyngeal carcinoma cell strain CNE-2Z / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the effects of procaine on human nasopharyngeal carcinoma cell strain CNE-2Z.@*METHOD@#CNE-2Z cells-cultured in vitro-were exposed to different concentration procaine and different time. Its growth characteristic were studied by using MTT assay and flow cytometry. The expression of p16 and RASSF1A mRNA were analyzed by RT-PCR.@*RESULT@#Procaine could inhibit the proliferation of CNE-2Z cells and the effects were in dose- and time-dependent manners. Procaine also arrested CNE-2Z cells in G1 phase of cell cycle. The expression of RASSF1A mRNA was up-regulated in CNE-2Z cells treated with procaine, but no obvious change was found in the expression of p16 mRNA.@*CONCLUSION@#Procaine could inhibit cell growth and up-regulate the expression of RASSF1A mRNA, which may play an important role in CNE-2Z cells' proliferation inhibition induced by procaine.

Up-regulation of the tumor-suppressing gene expression in nasopharyngeal carcinoma cells induced by Arsenic Trioxide / 中国耳鼻咽喉头颈外科

OBJECTIVE This study is to investigate the effects on nasopharyngeal carcinoma cell lines(CNE-2Z)induced by As2O3 and its possible mechanisms.METHODS CNE-2Z cells were treated with various concentrations of As2O3 for different times.The IC50 values were detected by trypan blue stain assay to choose the optimal concentration of As2O3.Cell cycle redistribution was analyzed by flow cytometry.The expression levels of p16、 RASSF1A、DNMT 3B mRNA were assessed by RT-PCR.RESULTS The suppression of cell proliferation by As2O3 was time and dose-dependent.After being treated with different concentration of As2O3,the IC50 values of As2O3 were(1.50?0.05),(1.09?0.13),(0.65?0.04)?mol/L at 24,48,and 72h,respectively.As2O3 also arrested CNE-2Z cells in G2/M phase of cell cycle.After CNE-2Z cells being treated with As2O3,the expression of RASSF1A mRNA were up-regulated(P

Expression of IFN-? and IL-12 in patients with allergic rhinitis / 中国耳鼻咽喉头颈外科

OBJECTIVE To explore the relationship between allergic rhinitis and IFN-?, IL-12. METHODS The expression of IL-12 was detected by in situ hybridization and enzyme-linked immunosorbent assay in 37 patients with allergic rhinitis and 12 control samples. IFN-? was detected by ELISA. RESULTS The positive ratios of IL-12 in PBMC were 0.13?0.03 and 0.77?0.15 in allergic rhinitis group and control group respectively. The concentrations of IL-12 were (13.7?2.4 ) pg/L and (52.9?8.4 ) pg/L in allergic rhinitis group and control group respectively. The concentrations of IFN-? were (11.2?2.1 ) pg/L and (51.8 ?6.4) pg/L in allergic rhinitis group and control group respectively. CONCLUSION The down-regulation of IL-12 and IFN-? was related to allergic rhinitis, and hyper-function of Th2 cells may play an important role in the pathogenesis of allergic rhinitis.

bcl-xs mediated sensitization of human nasopharyngeal carcinoma cells to camptothecin-induced apoptosis / 中国耳鼻咽喉头颈外科

Objective: In order to detect the effect of bcl-xs on camptothecin-induced apoptosis in human nasopharyngeal carcinoma CNE-2Z cells in vitro.Methods:bcl-xs gene-bearing mammalian expression vector(pcDNA3xs)was transfected into CNE-2Z cells using LipofectAmine.The expression of bcl-xs was determined with western blot.Cells which were transfected with native pcDNA3 vector were used as control.Apoptotic cells were detected with flow cytometry after exposure to camptothecin for 24h.Results:Cell clone(CNE-2Zxs)with stable expression of bcl-xs was obtained as confirmed with western blot.Results from flow cytometry analysis showed a significant increase of apoptotic cells in CNE-2Zxs as compared with CNE-2Zneo after treatment with the same dose of camptothecin.Conclusion:Exogenous bcl-xs expression sensitized nasopharyngeal carcinoma CNE-2Z cells to camptothecin-induced apoptosis.